Schofield, Andrew , Bi, Yong-Mei , Rothstein, Steven J .
Over-Expression of Monosaccharide Transporter Affects Growth of Arabidopsis thaliana Seedlings.
There are approximately 50 sugar transporters in Arabidopsis thaliana. Mutant studies have been unable to shed light on the function of most of these genes, perhaps because of functional redundancy within the families. This study uses constitutive over-expression of a monosaccharide transporter to understand the role of sugar transport in the growth of Arabidopsis thaliana. Several over-expression lines, created with the CMV 35S promoter, had increased levels of gene expression. Sugar uptake studies of 7-day old seedlings, demonstrated that over-expression lines had increased rates of 14C-glucose transport. After 10 days growth on AT media (9 mM nitrate) supplemented with either 55 mM glucose or 29 mM sucrose, over-expression plants were larger than Col-0 controls. There was no size difference when the media was not supplemented with sugar, suggesting that the increased rates of sugar uptake allowed the transgenic plants to grow faster. In terms of plant size, the over-expression and Col-0 plants responded similarly to changes in the growth conditions such as sugar levels (167 mM glucose or 88 mM sucrose), light levels (120 or 60 ╬╝mol m-2 s-1 PAR), and nitrate levels (low 3 or 1 mM nitrate); however, the over-expression plants were consistently larger than the Col-0 controls. This emphasizes the importance of sugar transport for seedlings growing on media.
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1 - University of Guelph, Department of Molecular and Cellular Biology, Guelph, Ontario, N1G 2W1, Canada
2 - University of Guelph, Department of Molecular and Cellular Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM