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Abstract Detail

Seed Biology

Naomab, Erold [1], Gatollin, Stefano [2], Alandete-Saez, Monica [3], Gonzalez-Carranza, Zinnia [4], Roberts, Jeremy [4].

The role of ARR22 and two-component systems during Arabidopsis pod development.

Response regulators are part of a multi-component phosphorelay system and are implicated in playing pivotal roles in modulating plant responses to critical environmental signals such as cytokinin and ethylene (Mizuno, 2005). ARR22 (At3g04280) is an atypical response regulator gene, whose function in Arabidopsis thaliana is unknown. Time course RT-PCR and Northern blot hybridization analysis reveal transcripts of ARR22 pre-dominantly in reproductive organs (Gattolin et al., 2006). Furthermore, β-glucuronidase (GUS) reporter gene expression driven by ARR22 promoter (ARR22::GUS) is specifically localized at the seed:funiculus junction. However, GUS activity is primarily visible when pods or seeds are mechanically wounded. Promoter analyses have shown no indication that the 183bp 5UTR intron regulates the wound inducibility of ARR22. Over-expression of ARR22 using the constitutive 35SCaMV promoter resulted in extreme dwarf transgenic phenotypes. ARR22 knock-out (KO) transgenic lines are phenotypically indistinguishable from wild type individuals under the conditions studied. Double knock-out lines of ARR22 and ARR24 are also similar to wild-type plants and whilst these two genes exhibit 66% amino acid similarity there is no evidence that they are functional homologous. Microarray approach has been followed to identify ARR22 co-regulated genes during pod development. ARRE is another gene having 75% amino-acid sequence similarity to ARR22 but which is structurally highly divergent, having two possible open reading frames. The two open reading frames were over-expressed separately using the constitutive 35SCaMV promoter. Homozygous 35S::ARRE transgenic lines have similar phenotypic characteristics to wild-type individuals.
Gattolin S, Alandete-Saez M, Elliott K, Gonzalez-Carranza Z, Naomab E, Powell C, Roberts JA (2006) Spatial and temporal expression of the response regulators ARR22 and ARR24 in Arabidopsis thaliana. J. Exp. Bot., 57(15): 4225 - 4233.
Mizuno T (2005) Two-Component phosphorelay signal transduction systems in plants: from hormone responses to circadian rhythms. Biosci. Biotechnol. Biochem. 69: 2263-2276.

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1 - University of Nottingham, Division of Plant Sciences, School of Biosciences, Sutton Bonington Campus, Loughborough, LE12 5RD, United Kingdom
2 - University of Birmingham, School of Biosciences
3 - Unversity of California, Berkeley, Department of Plant and Microbial Biology
4 - University of Nottingham, Division of Plant Sciences

response regulators
seed development

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P29026
Abstract ID:2283

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