Thomas, John , Zack, Colette , Stiney, Amanda , Liwienski, Amy , Elwahed, Abd .
Chino del Tomate Replication.
Chino del Tomate is a New World geminivirus that causes significant economic loss. This plant pathogen has two single stranded DNA genomes (CdTV-A and CdTV-B). The A genome is thought responsible for replication and viral packaging while the B genome encodes a nuclear movement protein and a cell-to-cell movement protein. Recovery of both the CdTV-A and CdTV-B genomes were facilitated by polymerase chain reaction (PCR), the genomes sequenced and the ORFs predicted. Partial dimers of the CdTV-A genome were constructed and inserted into the T-DNA vector pBin 19. Neither T0 nor T1 stable tobacco plant transformants demonstrated typical geminivirus symptoms (stunted growth, chlorosis, and twisting and curling of leaves). Two of five T1 plants showed virus replication and several leaves from the same plant demonstrated different amounts of viral DNA. Selected stable T0 tomato plants (cv. Alisa Craig) were found to demonstrate both severe symptoms and viral replication, noteworthy in the absence of a CdTV-B genome. Analysis of the T1 generation is ongoing. Efforts are focused upon is whether the CdTV-A genome can be stably incorporated into tomato, and then pass the ability of the CdTV-A virus to replicate in T1 progeny. Does the CdTV-A genome independently escape from the genome of numerous somatic cells in T1 plants or do one or a few viral escapes become packaged and spread through the plant in the absence of the movement proteins (BR1 and BL1). These results will assist in the future design of CdTV anti-viral strategies.
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1 - University of Michigan-Dearborn, Natural Sciences, 4901 Evergreen Rd, Dearborn, MI, 48128, USA
2 - University of Michigan-Dearborn, Natural Sciences
3 - University of Michigan-Dearborn, Department of Natural Sciences
4 - Agricultural Genetic Engineering Research Institute, Giza, EGYPT
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM