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Abstract Detail

Cell Walls

OBrien, Brent [1], Koch, K.E. [2], McCarty, Donald [2], Carpita, Nicholas C [3], Settles, Mark [2], Vermerris, WE [4], Avigne, W.T. [2], Hannah, Curt [2], McCann, Maureen [5].

Identification and Characterization of Selected Maize Cell Wall Mutants Generated in the UniformMu Population.

The plant cell wall is an intricate composite of diverse carbohydrate-based compounds. How these components are synthesized and integrated affects plant biology overall and also our capacity to effectively utilize cell walls as a renewable energy source. The distinctive Type-II cell wall of maize and other grasses is especially important, and differs markedly from that of Arabidopsis and other dicot species. For maize, the transposon-mutagenic Uniform Mu population allows knock-out mutations to be identified, localized to a specific gene, and studied in a uniform, inbred background. Here, a database of randomly-sequenced Mu-flanks was used to identify maize lines carrying mutations in genes for cell-wall biosynthesis. In addition, we have screened Mu-flank libraries constructed from knock-out mutants with kernel phenotypes. We are now testing progeny of these plants for co-segregation of phenotypes and knock-out mutations in specific genes. Thus far, we have identified and confirmed homozygous mutant lines for a cellulose synthase (CES), a polygalacturonase, a β-galactosidase, a glucose 4,6-dehydratase, and an arabinogalactan-protein encoding gene. Phenotypic features are being examined under field conditions. Understanding which cell wall genes are required for normal seed development will prove vital to future efforts to develop crops that maintain normal seed production while producing biomass that can be used efficiently.

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1 - Unifersity of Florida, PMCB, 1301 Fifield Hall, University of Florida, Gainesville, FL, 32611, USA
2 - University of Florida, Horticultural Sciences
3 - Purdue University, Dept. of Botany and Plant Pathology
4 - University of Florida, Agronomy
5 - Purdue University, Biological Sciences

cell wall

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P17046
Abstract ID:2253

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