Zhang, Qin , Riechers, Dean .
Proteomic identification of safener-induced proteins in Triticum tauschii seedling tissues and functional analysis of safener-induced glutathione S-transferases in aneuploid wheat lines.
Herbicide safeners protect cereal crops from herbicide toxicity, thereby providing a unique model system to study plant responses to a toxic chemical as well as a chemical that alleviates herbicide toxicity. Proteomic methods were used to identify safener- and/or herbicide-regulated proteins in three tissues of Triticum tauschii seedlings to gain a better understanding of safener mechanism of action. Growth experiments showed that the safener cloquintocet-mexyl protected seedlings from injury by the herbicide dimethenamid. In total, 29 safener-induced and 10 herbicide-regulated proteins were identified by LC-MS/MS. Surprisingly, mutually exclusive sets of proteins were identified following herbicide or safener treatment, suggesting that different signaling pathways may be recruited. Safener-responsive proteins are mostly involved in xenobiotic detoxification and metabolism, whereas most of the herbicide-regulated proteins are involved in general stress responses. A novel finding resulting from this study was that three isoforms of 12-oxophytodienoic acid reductase (OPR) proteins, which are involved in the biosynthesis of jasmonates, were highly induced by safener treatment. These proteomic results indicate that safeners protect T. tauschii seedlings from herbicide toxicity by inducing proteins involved in an entire herbicide detoxification pathway mainly in the coleoptile and root, thereby protecting new leaves from herbicide injury. Functional analysis of safener-inducible GST proteins in different aneuploid wheat lines (e.g., chromosome addition, deletion lines, or substitution lines) indicated that GST proteins TtGSTU1 and TtGSTU2 on chromosome 6DS confer safener-induced tolerance to dimethenamid in cultivated hexaploid wheat (Triticum aestivum).
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1 - University of Illinois at Urbana-Champaign, Crop Sciences, N-335, 1102 S. Goodwin. Ave, Urbana, IL, 61801, USA
2 - University of Illinois at Urbana-Champaign, Crop Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM