Growth and Vegetative Development
Woodward, Andrew W. , Lee, Jinsuk J. , Han, Zhiguo , Yang, S. Samuel , Chen, Z. Jeffrey .
Genetic And Functional Genomic Analyses Of Early Events In Cotton Fiber Development.
Cotton, an allotetraploid crop, is the major source of natural textile fiber. In addition, cotton fibers serve as an excellent model for understanding cell fate determination, rapid cell elongation, and cell wall biosynthesis. Each cotton fiber is a single differentiated protodermal cell of the ovule. Approximately 500,000 fibers are produced per cotton boll, some forming fuzz (short fibers) and others forming lint (long fibers of commercial value). Differentiated fiber cells visibly emerge from the ovule epidermis on the day of flowering (0 days post-anthesis, or DPA). Fiber initiation and development are affected by ploidy, pollination, fertilization, phytohormone treatment, and certain genetic mutations. However, it is unclear how these factors control the gene expression changes that orchestrate the pattern and tempo of fiber development. We are employing genetic and functional genomic approaches to investigate molecular events concomitant with fiber cell initiation. Comparison of a fiber mutant with wild type using spotted oligo arrays revealed differential accumulation of many transcripts, including MYB transcription factors and apparent phytohormone response regulators. Further, construction of a large EST library revealed an enrichment of specific functional categories of transcripts, as well as A-subgenome-derived transcripts, in young cotton ovules. Further expression and functional characterization will uncover transcripts that regulate cotton fiber initiation and development.
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The Z. Jeffrey Chen Laboratory
1 - The University of Texas at Austin, Institute for Cellular and Molecular Biology, 1 University Station A4800, Austin, TX, 78712, USA
2 - The University of Texas at Austin, Institute for Cellular and Molecular Biology
3 - Texas A&M University, Soil and Crop Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM