Protein Modification and Turnover
Hotton, Sara , Bostick, Magnolia , Callis, Judy .
Examination of the Arabidopsis RUB E1.
Protein degradation is essential for cellular and organismal homeostasis, and thus is under tight regulation. Eukaryotes share a highly conserved degradation system, the Ubiquitin (UBQ)-26S Proteasome System, or UPS. The UPS attaches UBQ, a small modifier protein, to target proteins via a conserved E1, E2, E3 cascade. Poly-ubiquitinated targets are then degraded on the proteasome [Annu Rev Plant Biol. 55 (2004): 555-590]. The RUB/Nedd8 (Related to UBiquitin/NEuronal-precursor cell Developmentally Down-regulated 8) pathway is integral to proteolysis, as it regulates the activity cullin-RING UBQ E3 ligases (CRLs) by attachment of RUB to the cullin subunit of the CRLs [Nature Rev Mol Cell Biol. 5 (2005): 9-20]. There are two Arabidopsis genes involved in the activation of RUB, namely AXR1 (AuXin Resistant 1), whose product functions as a heterodimeric RUB E1 with ECR1 (E1-C-terminus Related 1), and the related gene, AXL1, or AXr1-Like 1 [Plant Cell. 14 (2002): 421-433]. Several axr1 mutants have been characterized in Arabidopsis, whereas AXL1 has not been characterized. Here, we examine the function of AXL1 through characterization of an axl1 mutant and its relationship to axr1 mutants.
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1 - University of California, Davis, Molecular and Cellular Biology, 1 Shields Ave, Davis, CA, 95616, USA
2 - University of California, Los Angeles
3 - University of California, Davis
RUB activating enzyme.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM