Cheong, Eun Ju , Mock, Raymond , Li, Ruhui .
Optimizing the culture medium for meristematic tissue culture of several Saccharum species.
Meristematic tissue (MT) culture is commonly used to eliminate plant viruses from sugarcane plants, and success of regeneration from meristems on the reported media varies with genotypes. The aim of this study was to optimize nutrient concentrations in a medium to allow for growth of a wide range of Saccharum species and cultivars that enter the US through quarantine. Various concentrations of two macro-nutrients, nitrogen (N) and phosphorous (P), were examined using MTs of a cultivar (CP89-2143) and four species (S. barberi, S. officinarum, S. sinense, and S. spontaneum) that make up the genetic background of many US commercial sugarcane cultivars. The growth of MTs in liquid media was measured by fresh weight. The results show that changes to the concentration of N had a greater influence on regeneration of explants when compared to that of P. MTs of all tested species required at least 16 mM of total N for regeneration with a growth increment observed at 40mM of total N. Optimal concentrations of total N ranged from 40 mM to 66 mM for the tested species. The results show the hybrid cultivar has a more vigorous response to lower levels of N than the four species individually, with MT starting to regenerate at concentrations as low as 8mM of total N. A narrow range of total P was found to affect regeneration from MTs. Regeneration began even without P, but was suppressed at a concentration of 2.5 mM. When media were devoid of N and P, plant growth regulators (PGRs) such as BA, GA3 and kinetin had no effect on regeneration of MT. To further improve regeneration of MT of various species and cultivars, optimal PGRs and nutrient concentrations will be determined in future research.
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1 - USDA-ARS, Plant Science Institute, National Germplasm Resources Laboratory, Plant Disease Research Unit, 10300 Baltimore Ave. Buld.004 #025, beltsville, MD, 20705, USA
meristematic tissue culture
Presentation Type: Poster:Posters for Sections
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM