Dong, Shujie , Shew, David , Tredway, Lane , Lu, Jianli , Sivamani, Elumalai , Miller, Eric , Qu, Rongda .
Resistance of transgenic tall fescue to brown patch and gray leaf spot diseases.
Tall fescue (Festuca arundinacea Schreb.) is an important turf and forage grass species worldwide. Fungal diseases present major limitations to the maintenance of lawns and landscapes in the transition zone of the United States. The most common and severe diseases of tall fescue are brown patch, caused by Rhizoctonia solani, and gray leaf spot, caused by Magnaporthe grisea. In an attempt to obtain tall fescue with resistance to these diseases, four genes that have been associated with fungal disease resistance were introduced into the elite tall fescue cultivars ‘Coronado’ and ‘Matador’ of tall fescue through Agrobacterium-mediated transformation. The bacteriophage T4 lysozyme gene, the alfalfa Î²-1,3 glucanase AGLU1, and a truncated frog dermaseptin SI conferred resistance to both diseases, and the rice Pi9 gene showed resistance against gray leaf spot. In replicated experiments under controlled environments conducive to disease development, 12 of 28 T0 transgenic plants examined exhibited a high level of resistance to M. grisea, and six had enhanced resistance to R. solani. The resistance in most of these plants was highly significant. Five of the transgenic plants exhibited enhanced resistance to both diseases.
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1 - NC State University, Crop Science, 1126 Williams Hall, Raleigh, NC, 27695, USA
2 - NC State University, Plant Pathology
3 - NC State University, Crop Science
4 - NC State University, Microbiology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM