Szick-Miranda, Kathleen , Abidayo, Stacey , Zanial, Ali , Zanial, Ammar .
The Functional Significance of Evolutionarily Distinct Forms of Ribosomal Protein S15a in Arabidopsis.
The synthesis of proteins is an absolute requirement for growth and development in biological organisms, yet our understanding the core component (the ribosome) and its individual constituents (ribosomal proteins) in plants remains limited. The ribosomal protein (r-protein) S15a is encoded by six separate genes in the Arabidopsis genome. However, the products of those genes fall into two evolutionarily distinct categories (Type I and Type II). Recent analyses (Chang et al., 2005) have confirmed the presence of both Type I and Type II S15a within cytosolic ribosomes of Arabidopsis cell cultures. Our goal is to determine the functional significance of the divergent (Type II) forms of S15a in Arabidopsis. Examination of the expression of individual Type II S15a genes during various developmental stages by RT-PCR demonstrates that the two genes that encode Type II S15a are differentially expressed. In addition, we are utilizing a reverse genetics approach by examining mutations that disrupt the function of individual Type II S15a genes. Preliminary results indicate that the disruption of RPS15aB results in a severe developmental delay, similar to the minute phenotype described for (r-protein gene) mutations in Drosophila. Our results will add significantly to our understanding of the protein constitution of plant ribosomes and the functional significance of ribosome heterogeneity.
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1 - California State University, Bakersfield, Biology, 9001 Stockdale Hwy, Bakersfield, CA, 93311, USA
2 - CSU Bakersfield, Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM