Borisjuk, Ljudmilla , Neuberger, Thomas , Srenivasulu, Nese , Wobus, Ulrich , Rolletschek, Hardy .
Quantitative imaging of lipid storage in the developing seed.
Oil storage in plants is developmentally controlled, and undergoes multiple modulations within particular tissues. Understanding of the regulatory networks requires quantitative visualization data on storage lipid at a variety of stages and scales. We explored a rapid and non-invasive detection, visualization and quantification of lipid deposits in living seeds using frequency selected magnetic resonance imaging. Lipid deposition was characterized in vivo in two contrasting subjects: barley grains with a low, but highly compartmentalized, oil deposition, and soybean as an example of an economically important oilseed. Steep gradients in local lipid content were visualized in lipid maps that can not be obtained by any current method. Gradients were established within different organs of seed at the onset of storage, and were closely coordinated with seed maturation. In vivo lipid mapping coupled with gene expression analysis, biochemical measurements and electron microscopy demonstrated a high tissue-specificity of oil storage. The regions of maximum oil accumulation capacity can be easily defined without interrupting the developmental process. We suggest that application of lipid mapping on a wide range of plants may provide novel insights into the organization of lipid storage and is expected to facilitate genetic engineering and breeding of improved crops.
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1 - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Molecular Genetics, Correnstr. 3, Gatersleben, 06466, Germany
2 - Pennsylvania State University, Department of Bioengineering, University Park, Pennsylvania, 16802, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM