Doust, Andrew N , Koh, Jin , Tate, Jennifer A. , Paterson, Andrew H. , Soltis, Pamela S. , Soltis, Douglas E. .
Preferential gene loss or retention following genome duplication in allopolyploid species of Tragopogon (Asteraceae).
The vast majority of extant plant lineages shows evidence of at least one round of whole genome duplication. Such large scale duplications can act as a primary source of genes for new functions, and can thus be a major force in evolution. However, new functions take time to evolve and most variations that may lead to such functions are likely to be deleterious. Previous work in Arabidopsis, Oryza, Saccharomyces (yeast) and Tetraodon (puffer fish) [Paterson et al. 2006, Trends in Genetics] has suggested that genes with different protein functional domains may have different responses to duplication events, such that some remain as duplicated copies (that may then undergo divergence in function) while others rapidly lose a duplicate copy to become singletons again (“duplication resistant genes”). We are interested in the rate at which such divergence in gene fate occurs, and have been examining genes with different protein domains (as identified by Paterson et al.) in allopolyploid species of Tragopogon that have arisen within the last 80 years. We find rapid and random loss of gene copies for duplication resistant genes in individuals within the same allopolyploid populations, even within less than 40 generations. We are analyzing synthetic auto and allopolyploids from the same diploid parental species as the natural allopolyploids to ascertain how soon gene loss follows polyploidization. We are also analyzing expression data to determine whether gene loss is preceded by gene copy silencing. Initial findings indicate that gene loss can be extremely rapid, especially in whole genome duplications that result from allopolyploid events.
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1 - University Of Florida, Botany, PO Box 118526, Gainesville, FL, 32611, USA
2 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, Florida, 32611-8526, USA
3 - Massey University, Institute of Molecular BioSciences, Private Bag 11222, Science Tower D, Riddet Road, Palmerston North, New Zealand
4 - University of Georgia, Center for Applied Genetic Technologies, Plant Genome Mapping Laboratory, 111 Riverbend Rd., Athens, Georgia, 30606, USA
5 - University of Florida, Florida Museum of Natural History, PO Box 117800, Gainesville, FL, 32611-7800, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Lake Huron/Hilton
Date: Monday, July 9th, 2007
Time: 1:15 PM