Growth and Vegetative Development
Burkart, Graham , Harris, Jeanne M. .
A Screen for ABA-insensitive Mutants in Medicago truncatula.
The plant hormone Abscisic acid (ABA) mediates responses to abiotic stresses such as drought, cold and high salinity. Previous findings in our lab have shown abscisic acid to also play a role in nodule development as well as a novel role in lateral root growth in Medicago truncatula. Contrary to findings in Arabidopsis, ABA stimulates lateral root growth and formation in M. truncatula rather than inhibiting it. This suggests that ABA signaling in legume roots may be different than in non-legume roots. In order to better understand the function of ABA in nodulation and lateral root growth in M. truncatula, M2 seeds from an EMS-mutagenized population were screened for insensitivity to ABA for germination. We found a total of 61 putative ABA insensitive mutants representing 26 lines that germinated on media containing 50 uM ABA. We are currently re-screening the M3 generation. Plants that re-screen will have known and suspected ABA responsive genes sequenced and examined for mutations and the expression of these genes in the mutants will also be assessed. In addition, we will examine nodulation and lateral root growth. In this way, we hope to eventually determine how ABA functions in legume lateral root and nodule development.
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1 - University of Vermont, Plant Biology, 109 Carrigan Drive, Marsh Life Science Building, Burlington, Vermont, 05405, United States
2 - University of Vermont, Plant Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM