Protein Targeting and Vesicular Trafficking
Liu, Yang , Jiang, Liwen .
Molecular Characterization of Rice Vacuolar Sorting Receptor Proteins.
Plant cells contain both lytic vacuole and protein storage vacuole (PSV). Proteins reach vacuoles because they contain vacuolar targeting determinants that are recognized by vacuolar sorting receptor (VSR) proteins. BP-80, a VSR from pea, functions as a receptor mediating protein sorting from Golgi to prevacuolar compartment (PVC) for lytic vacuole. The transmembrane domain (TMD) and cytoplasmic tail (CT) of BP-80 were sufficient and essential to target a reporter protein via Golgi to lytic PVC. The Rice (Oryza sativa) genome comprises of five VSRs (OsVSR1-5) but little is known about their subcellular localizations and functions. Here we study the subcellular localization of OsVSR1-5 using a similar reporter approach. Toward this goal, we have generated transgenic tobacco BY-2 cells expressing GFP-OsVSR1-5 fusions TMD and CT sequences of individual OsVSRs. Confocal immunofluorescent and drug treatment studies demonstrated that these GFP-OsVSR fusions localized to either VSR-marked lytic PVC or Golgi apparatus. Their respective localization was further confirmed in rice cultured cells transiently co-expressed GFP fusions. Current efforts focus on studies of GFP-OsVSR1-5 fusions expressed in transgenic rice plants and their functions. This work was supported by grants from the Research Grants Council of Hong Kong (CUHK4260/02M, CUHK4307/03M and CUHK4580/05M), National Science Foundation of China (30529001), CUHK Scheme C and UGC-AoE (B-07/99) to L. Jiang.
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1 - The Chinese University of Hong Kong, Biology, Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, 000000, China
2 - The Chinese University Of Hong Kong, Biology
OsVSR Subcellular Localization GFP.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM