Trukhina, Yulia , Weber, Andreas .
Mutants of putative sugar transporters show altered carbohydrate levels in Arabidopsis thaliana.
There is a number of genes with putative sugar transporter function in Arabidopsis thaliana. Two of them are characterized in this study. Despite of high probability of plastidic localization the confocal microscope images of transiently transformed protoplasts show the Golgi localization of one of them - AtpGlcT (At5g16150). This correlates with the high similarity in N-terminal part between the AtpGlcT and Golgi-localized hexose transporter (AtSGB1). We speculate the temporarily Golgi-localization of the transporter targeted later to the plastids. The knock-out mutants of At5g16150 are smaller in size than the wild type plants if grown in the darkness. The double mutants of At5g16150 and At5g59250 (the other sugar transporter family protein) are smaller even under the normal conditions and show low myo-inositol and proline content. The mutants of At5g59250 show elevated free sugars and starch content and also chlorotic leaves if grown on sucrose under the long day conditions. The possible role of these two proteins is discussed.
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plant membrane protein database
1 - Michigan State University, Plant Biology, S-338 Plant Biology Bldg., East Lansing, MI, 48824, USA
2 - Heinrich-Heine-Universität Düsseldorf, Inst. für Biochemie der Pflanzen, Gebäude 26.03, Ebene 01, Universitätsstraße 1, Düsseldorf, 40225, Germany
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM