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Abstract Detail


Conservation Biology

Kim, Changkyun [1], Na, Hye-Ryun [1], Choi, Hong-Keun [1].

Genetic diversity and population structure of Korean Isoetes (Isoetaceae) using RAPD analysis.

Isoetes is an endangered aquatic plant in South Korea. We have investigated the genetic diversity within and between 10 populations of Isoetes found in South Korea by the method of randomly amplified polymorphic DNA (RAPD). Ten RAPD primers have produced a total of 94 bands, of which 71 (75.5%) are polymorphic. Genetic diversity is recognized within the populations of Korean Isoetes, as polymorphic loci (P) values range from 2.8% in the population of KWN to 28.2% in that of GNU. The UPGMA dendrogram for the analysis of the population genetic differentiation has revealed two distinct clusters of the genetic types: the mainland type and the Jeju Island. AMOVA analysis shows that genetic diversity is the highest among the populations (61.8%): within the populations (20.5%) and between two regions (the mainland and the Jeju island) (17.7%). These results indicate that the genetic differentiation has proceeded very fast. On the other hand, a rate of gene flow between the populations is as low as 0.061, irrespective of the distance between their genetic and geographical distances among the populations. Based on the present studies, we recommend the following as the conservation strategy for the species of Korean Isoetes: (1) The mainland type and the Jeju island are genetically distinct groups and so the conservation strategy for both type should be carried out independently. (2) To avoid any decline in genetic diversity caused by genetic drift and inbreeding, management units be considered to increase gene flow between the populations.


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1 - Ajou University, Division of Natural Sciences, Suwon, 443-749, South Korea

Keywords:
conservation
Endangered species
Isoetes
Genetic diversity
RAPD.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P65009
Abstract ID:1965


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