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Abstract Detail


Temperature Responses

Yu, Chih-Wen [1], Hung, Shu-Hsien [2], Yeh, I-Fang [2], Lin, Chin-Ho [3].

Hydrogen peroxide treatment induces cold tolerance in Arabidopsis via calcium-dependent signaling.

Hydrogen peroxide (H2O2) pretreatment effectively alleviated freezing injury in arabidopsis. Pretreatment with 1.0 mM H2O2 lowered the electrolyte leakage of arabidopsis chilled at -3°C for 6 h from 56% to 19% while, the electrolyte leakage of cold-acclimatized (at 4°C for 48 h) seedlings was found to be 16±3%, a trend very similar to that of H2O2 pretreatment (19±3%). Furthermore, after cold treatment, the growths of H2O2-treated and acclimated plants were remarkably less retarded compared with those of the control plants at normal temperature. Hydrogen peroxide induced freezing tolerance of arabidopsis seedlings was lowered by ethyleneglycol-bis(aminoethylether)-N,N´-tetraacetic acid (EGTA) treatment, but not by ruthenium red, indicating that the influx of Ca2+ from extracellular, but not intracellular, pools is an important signal in the induction of tolerance. Pretreatment of Ca2+-channel blocker lanthanum chloride and calmodulin and calcium-dependent protein kinases (CDPKs) antagonist W7 abrogated H2O2-induced freezing tolerance. In contrast, application of voltage-sensitive Ca2+-channel blocker nifedipine and calmodulin antagonist trifluoperazine exhibited no inhibitory effect on the H2O2-induced freezing tolerance. These results suggest that H2O2 pretreatment activates intracellular calcium signaling leading to the development of freezing tolerance in arabidopsis.


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1 - Department of Molecular Biotechnology, Da Yeh University, Chang Hua, Taiwan
2 - Department of Bioindustry Technology, Da Yeh University, Chang Hua, Taiwan.
3 - Department of Life Science, National Chung Hsing University, Taichung, Taiwan

Keywords:
Arabidopsis
Calcium
Cold
Hydrogen peroxide.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P08025
Abstract ID:1957


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