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Abstract Detail


Economic Botany: Applied Plant Biology

Teklu, Yifru [1], Karl, Hammer [2], Röder, Marion [3].

Comparative analysis of diversity indices and genetic relationships based on agronomic traits and microsatellites in tetraploid wheats.

Comparative analysis of genetic polymorphism and relationship in 133 Ethiopian tetraploid wheat accessions was conducted using morphological descriptors and molecular technique. Molecular variation was estimated using 29 polymorphic microsatellite loci, and quantitative variation was measured using 13 metric traits. Phenotypic and microsatellites diversity were estimated using the Shannon Weaver and Nei gene diversity indices, respectively. Diversity index estimated based on the entire quantitative and microsatellites data is 0.79 and 0.71, respectively. The Nei’s coefficient of gene differentiation (Gst) was equal to 0.15 for both phenotypic and microsatellites data implying that the genetic variation within and among the 11 geographical regions was 85 and 15%, respectively. Euclidean and Nei genetic distance estimates were calculated from binary phenotypic and molecular data matrices, respectively. The relationships between the two distances were assessed using simple linear correlation, mantel test, cluster analysis and principal component analysis. Results from the Mantel test and simple linear correlation analysis proved that the genetic variability based on microsatellites is not significantly correlated with the variability based on morphological characters. When the two distances were plotted against each other, the resulting scatter plot depicted the presence of a triangular shaped relationship between them. Both cluster diagram and bi-plot of the first two axis of PCA showed the presence of different patterns of geographical variation between microsatellites and phenotypic evaluations. The lack of concordance between molecular and quantitative measures of genetic variation suggests molecular measures of genetic diversity cannot exactly predict or substitute quantitative genetic variability.


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1 - 101 Curl Dr, Room 603, Columbus, OH, 43210, USA
2 - University of Kassel, Steinstr 19., Witzenhausen, Hessen, 37210, Germany
3 - Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, Gatersleben, Sachsen Anhalt, 06466, Germany

Keywords:
Genetic diversity
Landraces
wheat.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P66004
Abstract ID:1956


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