Coneybeer, Meagan M. , Hauk, Warren .
An Assessment of Genetic Variation within Cheiroglossa palmata (Ophioglossaceae) Using ISSR-PCR: Implications for Conservation.
Cheiroglossa C. Presl, commonly called the hand fern, is monotypic and one of only a few members of Ophioglossaceae that is largely epiphytic. Although C. palmata is distributed across the neotropics and more rarely the paleotropics, its range in continental North America is restricted to peninsular Florida. Florida populations of C. palmata are unique because they survive in a habitat considered marginal for the species, and are of conservation concern because extensive habitat destruction has reduced sizes and numbers of populations. Inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) was employed to analyze genetic variation among 147 sporophytes representing five Florida populations of C. palmata. Three (UBC-815, UBC-842, and UBC-845) of 100 ISSR primers surveyed produced both variable and reproducible banding patterns. Neighbor Joining and UPGMA revealed a total of 21 multilocus genotypes distributed among the sporophytes surveyed. Individuals sharing a single host tree did not always possess identical multilocus genotypes. Thus, wind dispersal of spores likely explains the origin of multiple multilocus genotypes on a single host tree. Bayesian analyses for dominant markers (Hickory 1.0) produced a θB of 0.35 and an ƒ value of 0.41, suggesting a moderate level of differentiation among the populations sampled and a mixed mating system. The Tosohatchee State Reserve populations, which contained the highest number of multilocus genotypes and the highest number of genotypes per population, are of highest conservation value. Establishment of in situ sites to protect the species and its habitat may facilitate the conservation of the species.
Log in to add this item to your schedule
1 - North Carolina State University, Department of, North Carolina State University, Raleigh, NC, USA
2 - Denison University, Department of Biology, 100 W College Street, Granville, Ohio, 43023, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Lake Michigan/Hilton
Date: Monday, July 9th, 2007
Time: 11:45 AM