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Abstract Detail


Water Relations

Sugawara, Yasutake [1], Htanaka, Rie [2], Sayama, Hideaki [2], Amagai, Hideki [2].

Desiccation tolerance of protoplasts and their regenerated cells of Marchantia polymorpha L.

In previous study, we have shown that cultured cells of M. polymorpha fully developed their desiccation tolerance when they were precultured on a medium with 0.5 M sucrose. These precultured cells were desiccated on silica gel and the content of water of these cells decreased below 0.1 gH2O/gDW after desiccation for 24 hrs. These desiccated cells were shown to vitrify at a temperature between 10 to 200C. More than 90% of the cells survived and grew vigorously on a regrowth medium after desiccation for 24 hrs. In the present study, we tried to induced the desiccation tolerance of protoplasts and their regenerated cells of M. polymorpha. Protoplasts were isolated from suspension-cultured cells that had been cultured in a medium with 0.5 M sucrose. Regenerated cells were obtained as follows: protoplasts isolated from M. polymorpha cells cultured in a standard medium were cultured for 24 hrs (more than 90% of the protoplasts regenerated new cell walls). These regenerated cells were transferred to the medium with a high concentration of sucrose and then cultured for 2.5 days. The isolated protoplasts and regenerated cells were embedded in 3% agarose gel and then desiccated on silica gel for 48 hrs. Survival rate of the protoplasts and regenerated cells was determined with FDA staining method and from regrowing rates of them after desiccation. Very high survival rates (>80%) were observed in the protoplasts and regenerated cells even after desiccation for 48 hrs.


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1 - Saitama University, Department of Regulation-Biology, 255 Shimo-okubo, Sakura-ku, Saitama, 338-8570, Japan
2 - Saitama University, Department of Regulation-Biology

Keywords:
none specified

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P09021
Abstract ID:1950


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