| Abstract Detail
Zalapa, J.E. [1], Brunet, Johanne [2], Guries, R. [3]. Hybridization between red elm (Ulmus rubra Muhl.) and Siberian elm (Ulmus pumila L.) confirmed using microsatellite markers. Ulmus pumila (Siberian elm) is an invasive elm species, non-native to the United States, which hybridizes with Ulmus rubra (red elm), a U.S. native. In order to study the population genetic structure of each species and the genetic hybridization patterns between the two species, we developed 38 primer pairs for microsatellite loci in U. rubra and tested their cross-amplification in U. pumila original accessions from China housed at the University of Wisconsin-Madison Elm Arboretum. In addition, eleven primer pairs previously developed in U. laevis and U. carpinifolia (syn. U. minor) were tested for cross-amplification. Sixteen primers amplified in both species, and thirteen loci proved polymorphic in U. pumila and 13 in U. rubra, each with 2 to 19 alleles per locus. Eight loci possessed species-specific alleles that clearly allowed the discrimination between U. pumila and U. rubra. First, we examined the genetic composition of 5 U. rubra populations and 53 U. pumila accessions original from China. Second, we determined the genetic basis of 6 Wisconsin populations containing U. pumila and U. rubra and intermediate phenotypes (i.e., putative hybrids). We aim to determine whether U. rubra is being assimilated genetically by DED-tolerant, U. pumila via this hybridization process. Results will help the development of conservation strategies for the long-term conservation of U. rubra and other elm native elm species in the U.S.
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1 - University of Wisconsin, USDA-ARS, Department of Horticulture, Department of Forest Ecology and Management, 1575 Linden Dr., 1630 Linden Dr., Madison, WI, 53706, USA
2 - USDA-ARS, University of Wisconsin, Department of Horticulture, 1575 Linden Drive, Madison, Wisconsin, 53706, USA
3 - University of Wisconsin, Department of Forest Ecology and Management, 1630 Linden Dr., Madison, WI, 53706, USA
Keywords:
hybridization
SSR
cross- species amplification
introgression.
Presentation Type: Array
Session: TBA
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: 006
Abstract ID:1937 |
