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Abstract Detail


Temperature Responses

Doherty, C.J. [1], Van Buskirk, H. [2], Myers, S.J. [2], Thomashow, Michael [2].

Identification of transcriptional activators of CBF2 in response to low temperature.

The CBF cold response pathway has an important role in cold acclimation, the process whereby plants increase in freezing tolerance in response to low nonfreezing temperatures. In Arabidopsis, a cold acclimating plant, three AP2 domain-containing transcription factors, CBF 1, 2, and 3 (DREB 1B, C, and A, respectively), are induced rapidly in response to low temperatures. Induction of CBF transcription factors is followed by expression of the CBF target genes, resulting in increased freezing tolerance. A key objective is to determine how plants sense low temperature and activate expression of the CBF genes. Our aim is to identify cis-acting promoter sequences and trans-acting proteins involved in controlling cold-responsive transcription of CBF2. We compared a 155 bp cold-responsive region of the CBF2 promoter with a 308 bp cold-responsive region of another cold-induced gene, ZAT12, and found several blocks of conserved sequences. Gene fusion studies indicated that a fragment containing two of the conserved sequences was sufficient to drive cold-responsive transcription in transgenic Arabidopsis. We identified a family of putative transcription factors that can bind to the conserved sequences. T-DNA insertion lines of these candidate factors were tested for their effect on the cold-induction of CBF2. T-DNA insertion in one transcription factor significantly reduced CBF2 cold induction levels. Together, these results suggest that this transcription factor has a role in regulating the cold-responsive transcription of CBF2.


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1 - Michigan State University, Plant Research Lab, 310 Plant Biology, East Lansing, MI, 48824, USA
2 - Michigan State University, Plant Research Lab

Keywords:
cold acclimation
freezing tolerance
CBF.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P08024
Abstract ID:1908


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