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Abstract Detail


Growth and Vegetative Development

Becker, JŲrg D [1], Takeda, Seiji [2], Dolan, Liam [2], Feijů, Josť A [3].

Transcriptional Profiling of Arabidopsis Root Hairs and Pollen Defines an Apical Growth Signature.

Despite their different origin and function, both pollen tubes and root hairs share the same sort of apical growth mechanism, i.e., the spatially focused cell expansion at the very apex. Ion fluxes, membrane trafficking, the actin cytoskeleton and their interconnection via signaling networks have been identified as fundamental processes underlying this kind of growth. Several molecules involved in apical growth have been identified, but the genetic basis is far from being fully characterized. We have used Affymetrix Arabidopsis ATH1 GeneChips to obtain the expression profiles of isolated Arabidopsis root hairs and flow-sorted pollen grains. The two profiles overlap in the expression of 4898 genes, which corresponds to 42% of the root hair transcriptome and 76% of the pollen transcriptome, respectively. Our comparison with transcriptional profiles of vegetative tissues by principal component analysis and hierarchical clustering shows a clear separation of these samples comprised of cell types with diffuse growth from the two cell types with apical growth. 277 genes are enriched and 49 selectively expressed, respectively, in root hairs and pollen. From this set of genes emerges an apical growth signature containing novel candidate genes for apical growth determination.


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1 - Instituto Gulbenkian de CiÍncia, Centro de Biologia do Desenvolvimento, Rua da Quinta Grande No 6, Oeiras, 2780-156, Portugal
2 - John Innes Centre, Department of Cell and Developmental Biology, Norwich, NR4 7UH, United Kingdom
3 - Faculdade de CiÍncias, Universidade de Lisboa, Dept. Biologia Vegetal, Campo Grande, Ed.C2., Lisboa, 1749-016, Portugal

Keywords:
Apical Growth
pollen
root hair
transcriptome
GeneChip.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P26056
Abstract ID:1892


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