Growth and Vegetative Development
Jaganatha, Venkateswari , Husbands, Aman , Bell, Elizabeth M , Girke, Thomas , Springer, Patricia .
Whole Transcriptome Analysis Reveals a Role in Brassinosteroid Signaling for LATERAL ORGAN BOUNDARIES in Arabidopsis.
The LATERAL ORGAN BOUNDARIES (LOB) gene in Arabidopsis defines a new conserved protein domain (the LOB domain) present in 42 other Arabidopsis proteins. LOB is expressed in a band of cells at the adaxial base of all lateral organs formed from the shoot apical meristem and also at the base of the lateral roots. Using a Selection and Amplification Binding (SAAB) assay, we identified a conserved nucleotide sequence bound by the LOB domain. Electrophoretic Mobility Shift Assays confirmed that this sequence is bound by the LOB domain of LOB, and by other LBD proteins, supporting the hypothesis that the LBDs represent a novel class of DNA-binding transcription factors. Loss-of-function lob mutants exhibit subtle organ fusion defects, while ectopic expression of LOB leads to a dwarf phenotype, similar to brassinosteroid-deficient mutants. Using 35S:LOB-GR plant material, a microarray analysis was performed, and several putative target genes were selected for further study. Here we present preliminary results showing that LOB regulates genes involved in brassinosteroid signaling.
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1 - University of California, Riverside, Department of Botany and Plant Sciences and Center for Plant Cell Biology, 2211 Batchelor Hall, University of California, Riverside, Riverside, California, 92521, USA
2 - University of California, Riverside, Department of Botany and Plant Sciences and Center for Plant Cell Biology
3 - John Innes Centre, Norwich Research Park, Colney, Norwich, Norfolk, NR, Cell & Developmental Biology Department
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM