| Abstract Detail
Mechanisms of Gene Regulation Bajsa, Joanna [1], Agarwal, Ameeta [2], Duke, Stephen O. [1]. The effect of protein phosphatase inhibitor cantharidin on A. thaliana transcriptom. We determined changes in the transcriptome profile of A. thaliana plants treated with cantharidin. Cantharidin is a natural compound isolated from the blister beetle. It is a potent inhibitor of protein phosphatase 1 and protein phosphatase 2A (PP2A) which play important roles in signal transduction pathways. They regulate gene expression, cellular proliferation, cell differentiation, and apoptosis. PP2A is involved in cell to cell communication, control of hormonal regulation (ethylene and abscisic acid), regulation of carbon and nitrogen metabolism and response to stress. Twelve-day-old seedlings of A. thaliana were sprayed with 200 μM cantharidin and then harvested after 2, 10 and 24 h of treatment. This concentration of cantharidin slowed down plant development and caused a broad spectrum of transcriptional responses. Transcription profiling revealed 2399 genes with changed expression (1264 genes up-regulated and 1135 down-regulated) during at least one time point. This is approximately 10% of the 24,000 genes of A. thaliana. The Arabidopsis plants treated by cantharidin developed a phenotype similar to mutant rcn1. The gene rcn1 encodes the regulatory subunit A of PP2A. The mutation of rcn1 has decreased PP2A activity. PP2A interacts with CTR1 which is negative regulator of the ethylene response. Inhibition of PP2A activity causes constant expression of ethylene-dependent genes (2.9% of down-regulated genes) and genes related to stress (4.5% of up-regulated genes). This apparently explains why the transcriptome profile of the cantharidin-treated plants was like that for biotic and abiotic stress responses.
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1 - USDA, ARS, Natural Products Utilization Research Unit, University, MS, 38677, USA
2 - National Center for Natural Products Research, School of Pharmacy, University of Mississippi
3 - USDA, ARS, Natural Products Utilization Research Unit, University, MS, 38677, USA
Keywords:
protein phosphatase inhibitor
microarray
Arabidopsis thaliana.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36043
Abstract ID:1881 |
