Unable to connect to database - 19:58:55 Unable to connect to database - 19:58:55 SQL Statement is null or not a SELECT - 19:58:55 SQL Statement is null or not a DELETE - 19:58:55 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 19:58:55 Unable to connect to database - 19:58:55 SQL Statement is null or not a SELECT - 19:58:55

Abstract Detail


Mechanisms of Gene Regulation

Eckert, Helene [1], Clemente, Thomas E [2], Stacey, Gary [3], Shoemaker, Randy C. [4], Wang, Kan [5].

Isolation and characterization of a gene involved in soybean nodulation.

As part of the project aiming at mapping the T-DNA insertions of a transgenic soybean population, we isolated a line carrying an insertion by a gene displaying high similarity with Nodulation Signaling Pathway 2 (NSP2) proteins from Pea, Lotus and Medicago. NSP1 and NSP2 are putative transcription factors from the GRAS-family. In legumes, they are activated upon infection of the root with symbiotic nitrogen-fixing bacteria (Rhizobium). Recent work on Lotus suggests that NSP2 probably induces NIN (putative transcription factor) and LjENOD40-1 (early nodulin) genes, which are required for nodule initiation (Murakami et al., 2007, DNA Research, doi:10.1093/dnares/dsl017). The transgenic line appeared to have a delayed growth under normal condition, as compared to wild-type. Growth under nitrogen-free conditions revealed that the transgenic event displays nitrogen deprivation phenotype regardless of whether or not it has been inoculated with Bradyrhizobium japonicum. Upon infection, the transgenic root produced few small nodules, apparently non-functional. We have cloned the gene and are working towards full characterization and understanding its function in soybean nodulation process.


Log in to add this item to your schedule

1 - Iowa State University, Agronomy, G403 Agronomy Hall, Ames, IA, 50011, USA
2 - University of Nebraska Lincoln, Agronomy and Horticulture
3 - University of Missouri, Plant Microbiology and Pathology
4 - Iowa State University, USDA-ARS-CICG, Dep. of Agronomy
5 - Iowa State University, Agronomy

Keywords:
nodulation
Glycin max
NSP2
nitrogen fixation
Bradyrhizobium japonicum.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36042
Abstract ID:1874


Copyright © 2000-2007, Botanical Society of America. All rights