Lee, Je Min , McQuinn, Ryan , Cara, Beatriz , Fei, Zhangjun , Klee, Harry , Giovannoni, James J. .
Comparative analysis of transcriptome and metabolite profiles in tomato introgression lines to identify candidate regulatory genes.
Phenotypic variation of diverse metabolites including vitamins, minerals, and antioxidants in tomato fruit has and will continue to be utilized for crop improvement. Comparative analysis of metabolic and genome expression profiling data from isogenic wild species introgression lines provides a unique opportunity for exploring the phenotypic outcomes of defined areas of the genome and for generating candidate genes for regulation of complex biochemical traits. Analysis of a series of tomato introgression lines (ILs) containing chromosomal segments of Solanum pennellii in a S. lycopersicum background allowed us to identify a subset of ILs with significant and reproducible variation in carotenoid accumulation. Some of these ILs contain chromosomal segments that are not linked to carotenoid biosynthesis loci and may influence aspects of pathway regulation. Gene expression profiling has been conducted using the recently developed tomato oligoarray ‘TOM2’, which represents approximately 12,000 tomato unigenes. Through a combination of transcriptome and metabolomic analysis, we seek to identify regulatory elements impacting the carotenoid synthesis pathway as a component to a larger effort toward elucidating the regulatory mechanisms of additional nutrient and aroma and flavor pathways.
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tomato metabolite database
1 - Boyce Thompson Institute for Plant Research, Ithaca, NY, 14853, USA
2 - USDA-ARS, Plant, Soil, and Nutrition Laboratory, Ithaca, NY, 14853, USA
3 - University of Florida, Department of Horticultural Sciences, Gainesville, FL, 32611, USA
4 - Boyce Thompson Institute for Plant Research, USDA-ARS, Ithaca, NY, 14853, USA
tomato introgression line.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM