Koehler, Gage , Randall, Stephen .
Evaluating dehydrin expression in Arabidopsis thaliana.
Our research objective is to understand the biological role(s) of a unique class of proteins called dehydrins (dehydration induced). Part of their uniqueness is attributed to their rapid accumulation in plants that exhibit the greatest tolerance to drought, cold, and salinity stress. Dehydrins remain at high levels until the stress subsides, suggesting dehydrins have an important role for plant growth during adverse conditions. Dehydrin accumulation is also associated with internal water deficit stress occurring with seed desiccation. The reason why plants require dehydrin proteins is unknown. The plant model system, Arabidopsis thaliana, is being used to evaluate the requirement of dehydrins during environmental stress and development. There are eleven dehydrins in Arabidopsis thaliana. Transgenic DNA insertions in ten individual dehydrin genes have been acquired to evaluate the impact of plants lacking the ability to accumulate these specific dehydrins (knockouts). These dehydrins differ from one another in their transcript and protein expression profiles. It will be interesting to know if these distinguishing features attribute unique functions or perhaps allow similar functions for distinct tissue. Analysis of micro array data and promoter regions have been performed. This information is being implemented into designing experiments that will evaluate phenotypic differences among wild type and dehydrin knockouts.
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1 - Indiana University - Purdue University Indianapolis, Department of Biology, 723 West Michigan Street, Indianapolis, IN, 46202, USA
2 - Indiana University Purdue University Indianapolis, Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM