Integrative Plant Physiology
Walley, Justin , Gopalan, Banu , Coughlan, Sean , Dehesh, Katayoon .
Elucidation of the primary stress induced signal transduction pathways in Arabidopsis.
Plants are continuously exposed to a myriad of abiotic and biotic stresses. It is thought that detection of physical stress is central to mounting a response against a broad range of environmental stresses. Plants then tailor their response based on the source (cold, dehydration, insect or pathogen), magnitude and duration of the stimuli. With the exception of a few pathogen elicitor receptors little is known regarding the initial signal transduction machinery that is used to perceive stress signals. To begin unraveling the primary signaling cascade we have conducted a full genome microarray experiment and determined what transcripts are differentially expressed shortly after mechanical wounding in Arabidopsis. To gain further insight into the expression pattern of a select group of upregulated genes a wounding time course was conducted and assayed via qRT-PCR. Several genes identified as rapidly and transiently upregulated were confirmed in vivo using stable transgenic lines expressing transcriptional luciferase fusions. Additionally, we have performed comparative bioinformatic analysis between primary genes induced by wounding as well as by other stresses. This analysis indicates that physical stress does play a universal role in stress perception and will be useful in the identification of the initial stress response cascade.
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1 - University of California, Davis, Section of Plant Biology, One Shields Ave, Davis, CA, 95616
2 - Cleveland Clinic Foundation
3 - Dupont
4 - University of California, Davis, Section of Plant Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM