Puckette, M , Mahalingam, R .
Microarray analysis of gene expression in ozone exposed Medicago truncatula.
Ozone is a model abiotic elicitor of reactive oxygen (ROS) in plants. We have screened 38 accessions of the model legume Medicago truncatula for their responses to acute ozone (Puckette et al., 2007). The ozone-resistant JE154 and sensitive accession Jemalong were used for temporal gene expression profiling. Samples for microarray analysis were collected early during ozone treatment (1 hour), at the end of ozone fumigation (6 hours) and six hours after the end of treatment.
The gene expression profiles dramatically differed between the accessions at each of the tested time points. Analysis of enriched Gene Ontology categories among the differentially expressed genes in JE154 showed early up regulation of transcription factors, hormone signaling, and genes important for ROS scavenging. On the contrary, the ozone-sensitive accession did not show massive changes in gene expression at the early time point. Up regulation of genes important for ethylene signaling and oxidative cell death by six hours provides a rational basis for explaining ozone-sensitivity of Jemalong.
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1 - Oklahoma State University, Biochemistry and Molecular Biology, 355 Noble Research Center, Stillwater, Ok, 74074, USA
2 - Oklahoma State University, Biochemistry and Molecular Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM