Zhu, Ying , Wang, Yi , Yang, Huijun , Hua, Jian .
Identifying components in transcriptional response to temperature variation.
Temperature is an important environment factor that regulates plant growth and development. However, how temperature is sensed and how the signal is transduced to modulate various developmental and physiological processes is not well understood. To isolated components in transcriptional reprogramming during temperature responses, we are isolating cis-acting elements and signaling components using two approaches in Arabidopsis.
1. Isolation of a temperature sensitive cis-element. BAP1 is a negative regulator of cell death and defense responses and its transcript is responsive to many environmental stimuli including wounding, pathogen and temperature change. BAP1 is transcriptionally induced by a small decrease in temperature in a SA dependent manner. By fusing a series of truncated BAP1 promoter with the GUS reporter gene, we have identified cis-elements responsible for its temperature responsiveness. This fragment of 35bp contains no known cis-elements and it should serve as a new starting point to reveal temperature signaling.
2. Genetic screens for temperature response mutants. Transgenic plants with temperature responsive reporter genes have been mutagenized and screened for mutants defective in temperature responses. Several mutants have been isolated, and characterization and cloning of these genes should reveal molecular mechanisms underlying temperature responses in plants.
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1 - Cornell University, Plant Biology, 141 Emerson Hall, Cornell University, Ithaca, NY, 14853, USA
2 - Cornell University, Plant Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM