| Abstract Detail
Metabolism Steichen, Jon [1], Sharkey, Thomas D. [2]. Cytosolic Maltose Metabolism in Arabidopsis thaliana. Maltose metabolism in the cytosol of Arabidopsis thaliana is thought to occur by a complex mechanism involving a cytosolic soluble heteroglycan intermediate and at least two enzymes; a 4-α-glucanotransferase (DPE2) and a glucan phosphorylase (AtPHS2), also known as Pho2 in other plant species. An Atphs2 knock-out mutant has a much less severe phenotype than a dpe2 knock-out mutant, which has led to the speculation of a third enzyme capable of bypassing the AtPHS2 catalyzed reaction. An α-glucosidase, that is predicted to be cytosolic, was cloned and expressed in E. coli. It was found to have a strong preference for short maltodextrins over long polyglucans in vitro with the highest activity on maltotriose, and therefore probably does not act directly on heteroglycan in vivo. Additionally, recombinant DPE2 was further characterized and shown to have a strict requirement for the β-anomer of maltose.
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1 - University of Wisconsin-Madison, Botany, B214 Birge Hall, 430 Lincoln Dr., Madison, WI, 53705, united states
2 - University of Wisconsin-Madison, Botany
Keywords:
α-glucosidase
4-α-glucanotransferase
maltose metabolism.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P19005
Abstract ID:178 |
