Plant biotech & Risk Assessment
Correa, Ana Maria , Siritunga, Dimuth .
Simple sequence repeat (SSR) assessment of genetic diversity of 23 cassava (Manihot esculenta Crantz) cultivars from Puerto Rico.
Cassava (Manihot esculenta Crantz) is the most important staple crop in the humid tropics and is food for more than 800 million people (FAO, 2001). The genetic variation at 30 microsatellite loci was used to investigate the geographical origins of 23 cassava cultivars present in Puerto Rico. Plant materials are a collection of cassava from Corozal Experimental Station and a representative group used in previous studies from Colombia, Costa Rica, Guatemala, Mexico, Peru and Brazil. The SSR markers were chosen to represent a broad coverage of the cassava genome with moderate to high polymorphism information content (PIC) and robust amplification to study the genetic variation within the Puerto Rican collection and to evaluate the phylogenetic relationships between these cultivars and their cultivated relatives from primary and secondary diversity centers. Microsatellite marker data were automated for high-throughput analysis using a 4300 DNA analyzer (LI-COR, Lincoln, NE, USA). At the moment the 30 markers have been amplified satisfactorily and show variability in size pattern between samples. In the near future, the corresponding data analysis will be made to measure the genetic distances between accessions and to estimate the genetic diversity parameters. The importance of this project will be the sustainable management of the cassava Puerto Rican genetic resources.
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1 - University of Puerto Rico at Mayaguez, Department of Biology, Carr. 108 Barrio Miradero Km 1.3 Entrada al Zoológico Mayagüez, PR 00680, Mayagüez, PR, Puerto Rico, 00680, Puerto Rico
2 - University of Puerto Rico at Mayaguez, Department of Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM