Bailey, Tameka Arnett , Yang, Yinong .
Molecular and Functional Characterization of Ethylene-Responsive MAP Kinases Associated with the Rice Defense Response.
Ethylene (ET) and abscisic acid (ABA) signal crosstalk are involved in the rice defense response against the blast fungus (Magnaporthe grisea) and abiotic stress signal transduction. To better understand the molecular mechanism underlying the signaling process associated with ET-mediated rice blast resistance and ABA-mediated abiotic stress tolerance, we have studied the potential involvement of two ET-inducible mitogen activated protein kinases (OsMPK13 and OsMPK17). Rice blast infection as well as 1-aminocyclopropane-1-carboxylic acid (ACC), an ethylene biosynthetic precursor, was shown to induce the expression of both genes and the protein and kinase activity of OsMPK17. In addition, many transgenic rice lines with suppression of OsMPK13 or OsMPK17 or overexpression of OsMPK17 were generated via RNA interference or transgene overexpression. In comparison with the wild-type control plant, these transgenic rice lines exhibited altered sensitivity and response to ET, ABA, rice blast infection, drought and/or salt treatments. Our preliminary results suggest that OsMPK13 and OsMPK17 are involved in the ET- and ABA-mediated rice blast resistance and abiotic stress tolerance.
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1 - University of Arkansas, Department of Plant Pathology and Program in Cell and Molecular Biology, Fayetteville, AR, 72701, USA
2 - Pennsylvania State University, Department of Plant Pathology, University Park, PA, 16802, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM