Scott, Rodney , Gastony, Gerald , Weatherford, Jeremy , Nakazato, Takuya .
Characterization of Four Members of the Alpha-Tubulin Gene Family in Ceratopteris richardii.
Four members of the alpha-tubulin gene family of Ceratopteris richardii were identified from clones isolated from a cDNA library by use of an antibody-based screening method. These genes were sequenced and found to be fairly similar to each other in terms of their amino acid sequences, with their greatest diversity at the carboxy-terminal ends. BLAST comparisons found each of these four amino acid sequences more similar to an alpha-tubulin from a dicot, gymnosperm, or alga species than it was to any other alpha-tubulin sequence presently known from Ceratopteris or from the fern Anemia phyllitidis or the moss Physcomitrella patens. Genetic linkage mapping based on a population of nearly 500 Doubled Haploid Lines was able to position three or four members of this gene family on linkage groups 17, 24, and 28, respectively (two of the four observed polymorphic restriction fragments containing alpha-tubulin genes are either identical or map too close to each other on linkage group 17 to be distinguishable in map distance). Non-mapable monomorphic bands observed on probed Southern blots suggest that the alpha-tubulin gene family in this species is large, a condition that may be correlated with the large genome size and diverse life history constraints of this homosporous fern species. The findings described in this study suggest several new opportunities for research into the evolution, function, and regulation of the alpha-tubulin gene family in Ceratopteris.
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1 - Wheaton College, Department of Biology, 501 College Avenue, Wheaton, IL, 60187, USA
2 - Indiana University, Department of Biology, Jordan Hall, 1001 East Third Street, Bloomington, Indiana, 47405, USA
3 - Washington University, Department of Computer Science and Engineering, St. Louis, MO, 63108, USA
genetic linkage mapping.
Presentation Type: Oral Paper:Papers for Sections
Location: Lake Michigan/Hilton
Date: Monday, July 9th, 2007
Time: 3:00 PM