Chang, Hsiang , Chai, Yu-Chin , Clark, David G. .
Utilizing SAG12-kn1 Petunias to Investigate Possible Application of kn1 to Delay Flower Senescence.
Kn1 is a homeobox gene that encodes a transcription factor known to play a critical role in plant development. Previous research has shown that the introduction of the kn1 gene into transgenic plants produces a phenotype of delayed leaf senescence and altered whole plant morphology consistent with an effect on cytokinin synthesis or sensitivity. Subsequent use of the senescence associated gene 12 (SAG12) promoter to drive kn1 expression in transgenic petunia led to the production of plants with a delayed leaf senescence phenotype. In order to investigate the untility for using kn1 to delay flower and leaf senescence, further experiments were conducted. Transgenic lines of petunia have been identified with increased flower longevity matching the presence of the kn1 construct as confirmed by PCR. Pollinated SAG12-kn1 flowers senesce approximately 50% slower than wild type 'Mitchell Diploid' (MD) controls. RT-PCR analysis of Kn1 to investigate gene activity in corollas after pollination will be reported. Senescence-related gene expression analysis comparisons between SAG12-kn1 and MD flowers will also be discussed with regard to the possible application of kn1 in delaying flower senescence.
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1 - Chung Chou Institute of Technology, Department of Biotechnology, 6, Lane 2, Sec. 3, Shanchiao Rd., Yuanlin Changhua, 51003, Taiwan
2 - Chung Chou Institute of Technology, Department of Biotechnology
3 - University of Florida, Environmental Horticulture Department
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM