Yang, Xin , Stevenson, Lauren , Ramonell, Katrina .
Characterization of Arabidopsis receptor kinases involved in fungal innate immunity.
The ability to recognize general elicitors and rapidly initiate defense responses is integral to the basal resistance of plants to most potential pathogens. These responses can be activated upon recognition of elicitors such as bacterial flagellin, plant cell wall fragments (oligogalacturonides; OG) released by pathogen damage and chitin fragments generated from the cell walls of pathogenic fungi. It has been shown that the recognition of elicitors involves receptor-like kinases (RLKs). In Arabidopsis, RLKs represent are a large family of genes including more than 610 putative members. Recently several RLKs have been shown to play a critical role in pathogen recognition and plant innate immunity. We compared of publically available microarray data sets of Arabidopsis plants treated with Chitin, Flg22, and oligogalacturonides(OG). Our analysis showed that 141 genes were specifically induced and 279 genes were specifically repressed by chitin treatment. From these data we identified 26 LRR-RLK genes of interest and obtained Salk T-DNA insertional lines. Homozygous T-DNA insertional lines were tested for resistance to the powdery mildew pathogen, Erysiphe cichoracearum. Four of the RLK knock-out lines were identified as more susceptible to powdery mildew than Columbia wild-type. Experimental Data on the characterization of these genes will be presented.
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1 - University of Alabama, Biological Science, 1108 14th AVE APT 324, Tuscaloosa, AL, 35401, USA
2 - University of Alabama, Biological Science
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM