| Abstract Detail
Integrative Plant Physiology Khandelwal, Abha [1], Elvitigala, Thanura [2], Zhou, Yiyong [3], Israelow, Ben [4], Ghosh, Bijoy [2], Quatrano, Ralph S. [5]. Redox homeostatis in plants: The Arabidopsis transcriptome in response to photosynthetic stress using high light and DCMU. Sensors and regulatory circuits that maintain redox homeostasis play a central role in adjusting plant metabolism and development to changing environmental conditions. We are studying a redox control network in Arabidopsis in response to photosynthetic stress. In order to develop an understanding of the behaviour of the network towards redox perturbations, we subjected Arabidopsis leaf discs, independently, to two commonly used photosynthetic stresses, DCMU and high light (HL). Microarray analysis of temporal gene expression patterns after DCMU and HL treated Arabidopsis leaf discs reveals that 17% and 20% of Arabidopsis transcriptome are under DCMU and HL regulation, respectively, with 6% comprising the common subset defined as redox responsive transcriptome (RRT). Further functional categorization demonstrates involvement of redox regulation in virtually all cellular processes. T-DNA insertion line in one of the transcription factors, from RRT was obtained and shown to have different sensitivity to photosynthetic stress compared to the wild type. A preliminary redox network was generated using these data.
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1 - Washington University in St. Louis, Department of Biology, One Brookings Drive, Campus Box 1229, St. Louis, MO, 63130-4899, USA
2 - Washington University in St. Louis, Department of Electrical and Systems Engineering
3 - Washington University, Department of Biology, Rebstock, 1 Brookings Drive, Campus Box 1137, St Louis, Missouri, 63130, USA
4 - Washington University, Department of Biology, Campus Box 1229, Saint Louis, Missouri, 63130, U.S.A.
5 - Washington University in St. Louis, Department of Biology
Keywords:
sensors
control network
metabolism
redox
microarray
high light
DCMU
motif
pathway.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P04015
Abstract ID:1683 |
