Coenen, Catharina , Brazelton, Jessica N. , Hayden, Joshua , Restori, Katherine .
Tools for Assessing Auxin Involvement in Arbuscular Mycorrhiza.
Secretion of the plant hormone auxin by ectomycorrhizal fungi is well established; however, the potential role for auxin in arbuscular mycorrhiza (AM) is less clear, because AM fungi are multi-nucleate, obligate symbionts and thus less amenable to genetic analysis. To assess a potential role for auxin in AM symbiosis, we developed a bioassay system for auxin that is based on transgenic Nicotiana plumbaginifolia seedlings expressing luciferase or beta-glucuronidase reporter genes under the control of the auxin-responsive GH3 promoter. While the luciferase-based assay system reliably detects auxin concentrations down to one micromolar, liquid media from the root-free compartment of two-compartment Daucus carota-Glomus intraradices co-cultures failed to elicit detectable auxin responses in this system. To assess whether the fungus may only secrete auxin locally inside host roots, we are characterizing GH3 promoter activity in response to fungal colonization using both luciferase and beta-glucuronidase reporters.
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Coenen Lab Website
1 - Allegheny College, Biology
2 - Allegheny College, Biology, 520 N Main Street, Meadville, PA, 16335, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM