Mechanisms of Gene Regulation
Chen, Q. , Fung, R. W. M. , Qiu, Wenping .
Functional Analysis of Grapevine Transcription Factors VaERF4 and VaERF5.
Ethylene response factors (ERF) belong to a plant-specific transcription factor family and play pivotal roles in plantís adaptation to various types of stresses. Two ERF genes, VaERF4 and VaERF5, were isolated from a grapevine (Vitis aestivalis) and classified into the group III that were presumed to be involved in plantís responses to abiotic stresses. These two ERFs were then expressed in model tobacco (Nicotiana tabacum) plants for studying their putative functions. Phenotype and gene expression profiles of VaERF4- and VaERF5-transgenic tobacco plants were investigated by comparing with wild type and ő≤-glucuronidase gene-transgenic tobacco plants. The biomass of individual VaERF5-transgenic tobacco plant significantly reduced in comparison with other plants on agar plates supplemented with Murashige and Skoog (MS) basal salts. The salt tolerance study showed that VaERF5-transgenic tobacco plants had significant lower reduction in total biomass than other plants after growing on MS media supplemented with 300mM NaCl for 35 days. No significant difference was observed among plants in terms of resistance to the fungus Botrytis cinerea. To discover putative target genes that are subjected to transcriptional regulation by the two ERFs, we applied quantitative real-time polymerase chain reaction to determine expression levels of selected downstream genes. We found that the transcript of a tobacco osmotin gene increased only in VaERF5-transgenic tobacco plants while other genes did not exhibit significant change. These results suggested that VaERF5 may be involved in the regulation of grapevineís response to abiotic stresses.
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1 - Missouri State University, Agriculture, 9740 Red Spring Road, Mountain Grove, MO, 65711, USA
2 - Missouri State University, Agriculture
ethylene response factor.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM