Cytoskeleton Structure and Dynamics
Ruzicka, Daniel, R. , Kandasamy, Muthugapatti K. , McKinney, Elizabeth, C. , Burgos-Rivera, Brunilis , Meagher, Richard, B. .
Novel and Differential Expression of Ancient Subclasses of the Arabidopsis ACTIN DEPOLYMERIZING FACTOR Gene Family.
The Actin Depolymerizing Factor (ADF) gene family of Arabidopsis thaliana encodes eleven functional protein isovariants in four ancient subclasses. We report the characterization of the tissue-specific and developmental expression of all Arabidopsis ADF genes and the subcellular localization of several protein isovariants. The four subclasses exhibited distinct expression patterns as examined by qRT-PCR and histochemical assays of a GUS-reporter gene under the control of individual ADF regulatory sequences. Subclass I ADFs were expressed strongly and constitutively in all vegetative and reproductive tissues except pollen. Subclass II ADFs were expressed specifically in mature pollen and pollen tubes or root epidermal trichoblast cells and root hairs, and these patterns evolved from an ancient dual expression pattern comprised of both polar tip-growth cell-types, still observed in the monocot Oryza sativa. Subclass III ADFs were expressed weakly in vegetative tissues, but were strongest in fast growing, and/or differentiating cells including callus, emerging leaves, and meristem regions. The single Subclass IV ADF was constitutively expressed at moderate levels in all tissues including pollen. Immunocytochemical analysis with subclass-specific monoclonal antibodies demonstrated that subclass I isovariants localize to both the cytoplasm and the nucleus of leaf cells, while subclass II isovariants predominantly localize to the cytoplasm at the tip region of elongating root hairs and pollen tubes. The distinct expression patterns of the ADF subclasses support a model of ADFs co-evolving with the ancient and divergent actin isovariants.
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1 - University of Georgia, Department of Genetics
2 - University of Georgia
actin depolymerizing factor
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM