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Abstract Detail


Organelle Biology

Masanek, Axel [1], Granvogl, Bernhard [2], Dotzler, Nora [2], Ploescher, Matthias [2], Eichacker, Lutz Andreas [2].

Proteomics of the plant cell.

In this study we show that proteomics of the plant cell can be performed well by investigation of the protein composition from the cell organelles that compartmentalize the cell. Organelles are being identified here as the natural next level for proteomic analysis of the cell. Organelles form the basis for detailed analysis of cell physiology and metabolism in time and space. We show that we will be wise counselled to fractionate the cell into organelles first, before we identify soluble and membrane proteomes from plant organelles. Proteomes are being separated by centrifugation and native electrophoresis and are identified by mass spectrometry. Sample preparation after electrophoresis has been carried out using OMX-Technology.


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1 - Ludwig-Maximilians-Universität München, Dept Biology I, Menzingerstraße 67, Munich, 80638, Germany
2 - Ludwig-Maximilians-Universität München, Dept Biology I

Keywords:
proteomics
plant cell
organelles
membranes
native PAGE
mass spectrometry
OMX.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P18026
Abstract ID:1618


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