Woo, Dong-Hyouk , Park, Hee-Yeon , Lee, Nam-Hoon , Lee, Chin Bum , Moon, Byoung Yong , Moon, Yong-Hwan .
Identification and Functional Characterization of Genes Regulating Abscisic Acid Biosynthesis in Abiotic Stress Signal Transduction in Arabidopsis.
Plants are usually exposed to various abiotic stresses in nature. A plant hormone, abscisic acid (ABA), is increased under abiotic stresses such as drought, high salinity and etc, and plays a central role in controlling plant's responses to those stresses by inducing the expression of defense genes. In this study, to identify upstream genes regulating ABA biosynthesis involved in abiotic stress signal transduction, we selected Arabidopsis mutants that had showed changed promoter activity of a 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in ABA biosynthesis, and performed a map-based cloning of mutated genes. The selected mutants are classified into two groups such as higher and lower NCED promoter activity than control after drought treatment. Most of the lower mutants were sensitive to drought, whereas some higher mutants were resistant. We selected 10 lower and 4 higher mutants for further studies based on responses to drought and visible phenotypes. One of the lower mutants, 1-8-2-6 was sensitive to drought and methyl viologen (MV). We have carried out map-based cloning of the 1-8-2-6, and found that gene responsible for the 1-8-2-6 mutants is located between 2.3 Mb and 5.1 Mb on chromosome 2. More fine mapping results will be presented and discussed.
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1 - Pusan National University, Department of Biological Sciences, Busan, 609-735, Korea
2 - Pusan National University, Department of Biological Sciences
3 - Dong-eui University, Department of Molecular Biology
4 - Inje University, School of Biotechnology and Biomedical Science
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM