Mechanisms of Gene Regulation
Lee, Jae-Yong , Pai, Hyun-sook .
The WD40-repeat protein RAE1 is involved in mRNA export and cell proliferation control in higher plants.
The WD40-repeat protein RAE1 is a component of nuclear pore complex, and has been implicated in nuclear export of poly(A)+ RNA and in the mitotic checkpoint regulation in the animal system. In this study, we investigated cellular effects of RAE1 deficiency in plants by using virus-induced gene silencing (VIGS). VIGS of NbRAE1 encoding a RAE1 homolog of Nicotiana benthamiana resulted in growth arrest and severe leaf abnormality. The GFP fusion protein of NbRAE1 was targeted to the nuclear rim, consistent with the finding in animals. Based on the whole-mount in situ hybridization, depletion of NbRAE1 caused the accumulation of poly(A)+ RNA in the nucleus, indicating that NbRAE1 is involved in the mRNA export pathway in plants. Furthermore, NbRAE1 VIGS lines exhibited phenotypes of disturbed cell proliferation control, such as inhibited leaf and root growth and suppressed xylem formation in the stem. Flow cytometry analysis revealed that the enlarged leaf cells showed increased ploidy level, suggesting a role of NbRAE in the endocycle. Based on these results, we propose that NbRAE1 plays a critical role in the mRNA export and the cell division control in plants.
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1 - Yonsei University, Biology, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea
2 - Yonsei University, Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM