Plant biotech & Risk Assessment
Park, Hyang-Mi , Kim, Yul-Ho , Shin, Dong-Bum , Choi, Man-Soo , Lee, Jang-Yong .
Enhanced tolerance to drought and ozone stress in PsAPX transgenic rice.
Ascorbate peroxidases (APX) plays an important role for scavenging ROS by degrading H2O2 in plants. The use of genes corresponding to specific enzymes of the ROS-scavenging system for plant transformation can lead to the development of transgenic plants with enhanced tolerance to oxidative stress. Pea ascorbate gene (PsAPX) with the sequence coding for chloroplast targetting under the control of oxidative stress-inducible promoter, sweet potato peroxidase anionic 2 (SWPA2) was transformed into rice by Agrobacterium-mediated transformation. Transformation was confirmed by Southern blot analysis. Leaves of transgenic rice plants showed high-levels of PsAPX gene transcripts when they treated under drought and ozone stress. By exposing the transgenic plants to drought and ozone stress, the tolerance of transgenic plants was evaluated and confirmed by measuring ion leakage, chlorosis and relative water content in the leaf. These transgenic rice plants are also being tested for cold tolerance in the field condition. Our results suggest that PsAPX over-expression could improve abiotic tolerance by scavenging ROS.
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1 - National Institute of Crop Science, Environment and Biotechnology Division, Suin-ro, 150, Seodun-dong Gwonseon-gu, Suwon, Gyeonggi-do, 441-857, Korea
2 - National Institute of Crop Science, Environment and Biotechnology Division
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM