Sánchez-Calderón, L. , Lang, J. , Smetana, A , Montané, M.-H. , Houlné, G. , Chabouté, M.E. .
Novel aspects of nuclear foci formation in plant DNA damage response.
The ability of plants to respond to genotoxics which compromise genomic integrity is crucial to their survival and reproduction. Among the various DNA damage which could happen in the cell, the double strand break (DSB) is one of the most cytotoxic events. The cellular response to DSBs involves a large number of specific proteins responsible for damage detection, DNA repair, cell cycle arrest and resumption. When a DSB occurs, the histone H2AX is phosphorylated (gamma-H2AX) in the chromatin surrounding DSBs to form nuclear foci. In animals these foci represent active center for recruitment of DNA repair proteins. Recently, the discovery of gamma-H2AX foci in Arabidopsis suggests that some of DSB signalling might be conserved among the species. However the dynamic of interactions between these proteins gathered in the foci is still poorly documented. To gain knowledge on this topic, we generated several transgenic BY2 cells lines and Arabidopsis plants harbouring NtE2F as well as some Atlike-FHA-BRCT domain proteins. Strikingly in the DSB response, NtE2F formed foci colocolizing with gamma-H2AX. Our purpose is to look further for possible colocalization of E2F with our FHA-BRCT domain proteins of interest in DNA damage response.
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1 - Institut de Biologie Moléculaire des Plantes, Biologie cellulaire et développement des plantes, 12 rue du general Zimmer, Strasbourg, Strasbourg, 67084, France
2 - Institut de Biologie Moléculaire des Plantes, Biologie cellulaire et développement des plantes
3 - CEA, DSV, Institut de Biologie Environnementale et de Biotechnologie (, Service de biologie végétale et de microbiologie environnementales (SBVME), Saint Paul-lez-Durance, France
DNA double strand break
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM