Liu, Wen-Yu , Ho, Feng-Ju , Lin, Tsai-Yun .
Characterization of three ERF transcription factors from Bupleurum kaoi.
We isolated and identified three ethylene responsive transcriptional factors (ERF) genes and analyzed their expressions in various abiotic stress conditions. The three genes are classified as members of the ERF subfamily B-3 of ERF/AP2 transcription factor family based on multiple sequence alignment and phylogenetic characterization. Analysis of the deduced amino acid sequences revealed that the BkERFs contain a conserved ethylene-responsive element binding protein (EREBP/AP2) domain. The BkERF cDNAs were cloned in pET-32 Ek/LIC vector and transformed into Escherichia coli. Electrophoretic mobility shift assay (EMSA) showed that these three ERF proteins bind to the GCC box (an element responsive to ethylene). The quantitative real-time RT-PCR analysis indicated the BkERFs were induced by 1-aminocyclopropane-carboxylic acid and methyl jasmonate, not salicylic acid. Our results suggest that BkERFs might act as positive transcription factors in stress signal transduction pathways.
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1 - National Tsing Hua University, Institute of Bioinformatics and Structural Biology, No 101, Sec 2, Kuang Fu Road, Hsinchu, 30013, Taiwan, Republic of China
2 - National Tsing Hua University, Department of Life Science & Institute of Bioinformatics and Structural Biology, No 101, Sec 2, Kuang Fu Road, Hsinchu, 30013, Taiwan, Republic of China
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM