Chan, Yuan-Li , Chan, Ming-Tsair , Yeh, Kai-Wun .
Engineering nematode-resistant tomato with stacking of cystatin and chitinase genes.
Root-knot nematode is one of the most harmful pests in tomato and causing serious economic loss. Nematode-infected root tips become withered and decayed resulting in the formation of root knots, affects plant growth and development with reduced disease resistance and yield. Thus, we engineered nematode resistance in local commercially important heat-tolerant tomato cultivar CLN2468 by introducing two defense genes isolated from Paecilomyces javanicus and taro, respectively by gene stacking approach. To identify the most efficient promoter for the expression of target genes four different promoters were tested, including CaMV35S promoter, RB7 promoter, sporamin promoter and a synthetic promoter containing two elements of NOS motif fused with GUS gene and expressed in either hairy root or transgenic plant systems, correspondingly. Histochemical staining and GUS activity assay revealed that the synthetic promoter displayed constitutive and strongest expression than other promoters tested. However, sporamin promoter showed undetectable expression in transgenic hairy roots. In addition, there is no wounding effect on the gene expression observed in transgenic hairy roots harboring the synthetic promoter fused with GUS gene. These results suggest that the synthetic promoter can be the most efficient promoter to drive the target genes in transgenic tomato plants. Currently, transgenic tomato plants harboring these two genes under the control of the synthetic promoter and their nematode defense, and resistance mechanism is in progress.
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1 - Institute of Plant Biology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan
2 - Agricultural Biotechnology Research Center, Academia Sinica
3 - Institute of Plant Biology, National Taiwan University
root knot nematode
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM