Modeling and Computational Biology
Ilut, Daniel , Doyle, Jeff J. .
IntrEST - a database of highly conserved coding sequences containing putative intron splice sites.
IntrEST (http://intrest.pgn.cornell.edu) is a database of expressed sequence tag (EST) derived sequences for which we have inferred putative intron splicing sites using a comparative genomics approach. The database contains sequences sampled from over 50 phylogenetically diverse reference species, each providing hundreds of ready to use candidate sequences. For each reference species, sequences were selected that meet two criteria: 1) an intron splice site is present in homologous Arabidopsis thaliana genomic sequence, and 2) the site is flanked by sequences that are highly conserved between Arabidopsis and the reference species, of sufficient length that PCR primers can be constructed to amplify across the hypothesized intron. Such primers can be used to amplify the region across closely-related species in genera allied to the reference taxon.
Log in to add this item to your schedule
1 - Cornell University, L.H. Bailey Hortorium, Department of Plant Biology, Ithaca, New York, 14853, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM