Mechanisms of Gene Regulation
Huang, Yen-Chiao , Sun, Chih-Wen .
Regulating the expression of chloroplast translocon and ribosomal protein genes in Arabidopsis.
Chloroplasts, originated from a cyanobacterial ancestor, transfer the majority of their genes to the host nucleus during evolution. Therefore, plant nuclear genome plays an important role in regulating chloroplast development nowadays. Our previous studies found that CIA2 gene in Arabidopsis encoded a transcription factor which regulated the expression of chloroplastic translocon genes, atToc33 and atToc75. The subsequent microarray analysis also indicated that CIA2 modulated the expression of plastid-specific ribosomal genes. In order to investigate whether CIA2 binds to its downstream genes in vivo, chromatin immunoprecipitation technique is employed in this study to prove that CIA2 directly interacts with promoters of specific trnaslocon and plastid ribosomal protein genes in nucleus. Furthermore, the promoter structure of Arabidopsis CIA2 and its downstream genes are analyzed by bioinformatic programs in detail. We find two novel cis-acting elements existed in the promoter sequences of all CIA2-downsteam genes, and therefore named as CIARE1 and CIARE2 (CIA2-regulatory element). EMSA and ChIP experiments further indicate that CIA2 specifically binds to promoter sequences of its downstream genes. These data will not only reveal the pathway how CIA2 regulates the chloroplast genes, but also help in understanding the mechanism of chloroplast development.
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1 - National Taiwan Normal University, Department of Life Science, No.88, Sec. 4, Tingzhou Rd., Taipei, 116, Taiwan
2 - National Taiwan Normal University, Department of Life Science
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM